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IGVFDS2716FVEL

released
Aliases
charles-gersbach:cd8-tcells-intermediate-analysis-crispri-b2m
File Set Type
intermediate analysis
Summary
CRISPR interference FACS screen targeting B2M: alignments
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of B2M

Files

6 items
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
14 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
16 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
13 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
16 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
14 MB
validated
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
5.1 MB
validated

File Association Graph

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File Set
File
AnalysisSet
AuxiliarySet

Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-19
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-18
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-17
unspecified
Homo sapiens

AuxiliarySet Input File Sets

6 items
Associated Measurement Sets
Associated Construct Library Sets
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

File Sets Using This Analysis Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR interference FACS screen targeting B2M: fold change over control, guide quantifications
  • charles-gersbach:cd8-tcells-analysis-set-crispri-b2m
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke