IGVFDS2261TIMI

released

Aliases

charles-gersbach:jurkats-analysis-set-crispra-vp64-il2ra

File Set Type

principal analysis

Summary

CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications

Publications

Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens

Samples

Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA

Files

2 items

IGVFFI2207HLUJ

tsv

fold change over control

custom assembly fold change over control

Charles Gersbach, Duke

14 KB

validated

IGVFFI7694LAUN

csv

guide quantifications

custom assembly guide quantifications

Charles Gersbach, Duke

9.4 KB

validated

File Association Graph

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File Set

File

AnalysisSet

Samples

6 items

Sample Terms

Summary

IGVFSM1480DJBL

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM2737ATVK

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM3148FRBY

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM3606VJWU

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM4401YRWQ

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM7748IVLZ

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)