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IGVFDS0320JXJG

released
Aliases
charles-gersbach:cd8-tcells-analysis-set-crispra-ccr7
File Set Type
principal analysis
Summary
CRISPR activation FACS screen targeting CCR7: fold change over control, guide quantifications
Publications
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of CCR7

Files

2 items
tsv
fold change over control
custom assembly fold change over control
Charles Gersbach, Duke
73 KB
validated
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
48 KB
validated

File Association Graph

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File Set
File
AnalysisSet

Samples

6 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

3 items
Aliases
  • charles-gersbach:cd8-tcells-donor-20
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-22
unspecified
Homo sapiens
  • charles-gersbach:cd8-tcells-donor-21
unspecified
Homo sapiens

AnalysisSet Input File Sets

1 item
Accession
Summary
CRISPR activation FACS screen targeting CCR7: alignments

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke