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IGVFFI9097APHN

released
validated
File Set
IGVFDS5551EATG(CRISPR knockout FACS screen targeting IL7R: alignments)
Summary
custom assembly unfiltered alignments
Analysis Step Version
/analysis-step-versions/f9f6ae88-d515-4e49-9902-deea3cf87703/
File Format
bam
Content Type
alignments
Content Summary
unfiltered alignments
Aliases
charles-gersbach:cd8-tcells-alignment-crispr-ko-tfome-high-bin-rep1
md5sum
aa5f1c2e4afb818dc0c0ed222d8f2628
Content MD5sum
adef5dd01e70c4bf15fb5c325720befd
File Size
590 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/pSM73_H2_S5.sorted.bam

Alignment Details

Reference Files

Samples

4 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
fastq
reads
Charles Gersbach, Duke
856 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
147 KB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke