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IGVFFI8774OGSY

released
validated
File Set
IGVFDS5157TBYH(CRISPR interference FACS screen targeting CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2 with low FACS signal control)
Summary
custom assembly unfiltered alignments
Analysis Step Version
/analysis-step-versions/f9f6ae88-d515-4e49-9902-deea3cf87703/
File Format
bam
Content Type
alignments
Content Summary
unfiltered alignments
Aliases
charles-gersbach:cd8-tcells-alignment-crispri-cd2-low-bin-rep2
md5sum
8059b9c1052c09a0bd5e7aaf32e116f9
Content MD5sum
3e609d9a1418f6d5907f90a1b1200f97
File Size
19 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/pSM8-Low-R2_S13.sorted.bam
Checkfiles Version
Legacy version of checkfiles
Attribution
Charles Gersbach, Duke

Alignment Details

Reference Files
Genome Assembly
custom

Samples

4 items
Sample Terms
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
fastq
reads
Charles Gersbach, Duke
27 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
8.1 KB
validated