IGVFFI7996HHYF

released
validated
File Set
IGVFDS5478QWPN(CRISPR activation FACS screen targeting IL2RA: alignments)
Summary
custom assembly unfiltered alignments
Analysis Step Version
File Format
bam
Content Type
alignments
Content Summary
unfiltered alignments
Aliases
charles-gersbach:jurkats-alignment-crispra-vp64-il2ra-high-bin-rep2
md5sum
6b580342142fb0e393a49b1bf537a483
Content MD5sum
b98409114126624cbbc65bb6809585cc
File Size
20 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/pSM44-High-R2_S10.sorted.bam

Alignment Details

Reference Files

Samples

6 items
Sample Terms
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
fastq
reads
Charles Gersbach, Duke
29 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
9.4 KB
validated

Attribution