{
"@context": "/terms/",
"@id": "/alignment-files/IGVFFI7658XOUY/",
"@type": [
"AlignmentFile",
"File",
"Item"
],
"accession": "IGVFFI7658XOUY",
"aliases": [
"charles-gersbach:cd8-tcells-alignment-crispri-ccr7-high-bin-rep1"
],
"analysis_step_version": "/analysis-step-versions/f9f6ae88-d515-4e49-9902-deea3cf87703/",
"assay_titles": [
"CRISPR FACS screen"
],
"assembly": "custom",
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "6d610ede943c88fbc48f58a9988904ff",
"content_summary": "unfiltered alignments",
"content_type": "alignments",
"controlled_access": false,
"creation_timestamp": "2024-10-15T18:59:21.594747+00:00",
"derived_from": [
"/sequence-files/IGVFFI6405ADNH/"
],
"file_format": "bam",
"file_set": {
"@id": "/analysis-sets/IGVFDS9065THHI/",
"accession": "IGVFDS9065THHI",
"file_set_type": "intermediate analysis",
"samples": [
{
"@id": "/primary-cells/IGVFSM9398LTWW/",
"accession": "IGVFSM9398LTWW",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7413JCSK/",
"accession": "IGVFSM7413JCSK",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM2207SJEN/",
"accession": "IGVFSM2207SJEN",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM1205VMNO/",
"accession": "IGVFSM1205VMNO",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"summary": "CRISPR interference FACS screen targeting CCR7: alignments"
},
"file_size": 13972258,
"filtered": false,
"gene_list_for": [],
"href": "/alignment-files/IGVFFI7658XOUY/@@download/IGVFFI7658XOUY.bam",
"input_file_for": [
"/tabular-files/IGVFFI3289WICA/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "2f41318579ffb1e29296058830833419",
"read_count": 805013,
"redacted": false,
"reference_files": [
"/reference-files/IGVFFI6999YLZG/"
],
"release_timestamp": "2024-11-18T21:09:09.313176+00:00",
"s3_uri": "s3://igvf-files/2024/10/15/7ecff3f4-5e98-4d37-8121-c79cffe3f0f9/IGVFFI7658XOUY.bam",
"schema_version": "11",
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CCR7pos-pSM27-TF-H1_S2.sorted.bam",
"summary": "custom assembly unfiltered alignments",
"upload_status": "validated",
"uuid": "7ecff3f4-5e98-4d37-8121-c79cffe3f0f9"
}