IGVFFI6815BFHV

released
validated
File Set
IGVFDS2261TIMI(CRISPR activation FACS screen targeting IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA with low FACS signal control)
Summary
custom assembly unfiltered alignments
Analysis Step Version
File Format
bam
Content Type
alignments
Content Summary
unfiltered alignments
Aliases
charles-gersbach:jurkats-alignment-crispra-vp64-il2ra-high-bin-rep1
md5sum
e2e00ddb7c797224646b781e79dcc448
Content MD5sum
46a7ac8a5c8125ed6c797236ada8faca
File Size
20 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/pSM44-High-R1_S8.sorted.bam
Checkfiles Version
Attribution

Alignment Details

Reference Files
Genome Assembly
custom

Samples

6 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files This File Derives From

1 item
Accession
File Set
File Format
Content Type
Lab
File Size
Upload Status
fastq
reads
Charles Gersbach, Duke
28 MB
validated

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
custom assembly guide quantifications
Charles Gersbach, Duke
9.4 KB
validated