IGVFFI3587KQSB

released

validated

File Set

IGVFDS5478QWPN(CRISPR activation FACS screen targeting IL2RA: alignments)

Summary

custom assembly unfiltered alignments

Analysis Step Version

/analysis-step-versions/f9f6ae88-d515-4e49-9902-deea3cf87703/

File Format

bam

Content Type

alignments

Content Summary

unfiltered alignments

Aliases

charles-gersbach:jurkats-alignment-crispra-vp64-il2ra-low-bin-rep1

md5sum

d00d7411b2949872717b7dea3a044dcf

Content MD5sum

9dc4edb34eda44134bf43fcd78ed0cc1

File Size

17 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/pSM44-Low-R1_S7.sorted.bam

Alignment Details

Reference Files

IGVFFI5804OZJN

Samples

6 items

Sample Terms

Summary

IGVFSM1480DJBL

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM2737ATVK

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM3148FRBY

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM3606VJWU

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM4401YRWQ

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM7748IVLZ

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)