IGVFFI1153QAAC

released
validated
{
    "@context": "/terms/",
    "@id": "/alignment-files/IGVFFI1153QAAC/",
    "@type": [
        "AlignmentFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI1153QAAC",
    "aliases": [
        "charles-gersbach:jurkats-alignment-crispra-vp64-il2ra-high-bin-rep3"
    ],
    "analysis_step_version": "/analysis-step-versions/f9f6ae88-d515-4e49-9902-deea3cf87703/",
    "assay_titles": [
        "CRISPR FACS screen"
    ],
    "assembly": "custom",
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "219b010728eefc5a07f67f893e46d091",
    "content_summary": "unfiltered alignments",
    "content_type": "alignments",
    "controlled_access": false,
    "creation_timestamp": "2024-10-15T19:08:29.778508+00:00",
    "derived_from": [
        "/sequence-files/IGVFFI3109KTCA/"
    ],
    "file_format": "bam",
    "file_set": {
        "@id": "/analysis-sets/IGVFDS5478QWPN/",
        "accession": "IGVFDS5478QWPN",
        "file_set_type": "intermediate analysis",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM1480DJBL/",
                "accession": "IGVFSM1480DJBL",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "term_name": "Jurkat"
                    }
                ],
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM3148FRBY/",
                "accession": "IGVFSM3148FRBY",
                "classifications": [
                    "cell line"
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                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "term_name": "Jurkat"
                    }
                ],
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM3606VJWU/",
                "accession": "IGVFSM3606VJWU",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "term_name": "Jurkat"
                    }
                ],
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM7748IVLZ/",
                "accession": "IGVFSM7748IVLZ",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "term_name": "Jurkat"
                    }
                ],
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM4401YRWQ/",
                "accession": "IGVFSM4401YRWQ",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "term_name": "Jurkat"
                    }
                ],
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/in-vitro-systems/IGVFSM2737ATVK/",
                "accession": "IGVFSM2737ATVK",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002796/",
                        "term_name": "Jurkat"
                    }
                ],
                "summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "CRISPR activation FACS screen targeting IL2RA: alignments"
    },
    "file_size": 17967420,
    "filtered": false,
    "gene_list_for": [],
    "href": "/alignment-files/IGVFFI1153QAAC/@@download/IGVFFI1153QAAC.bam",
    "input_file_for": [
        "/tabular-files/IGVFFI7694LAUN/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "ff244cd816e8e25051df30ba775de484",
    "read_count": 1209431,
    "redacted": false,
    "reference_files": [
        "/reference-files/IGVFFI5804OZJN/"
    ],
    "release_timestamp": "2024-11-18T21:08:55.344520+00:00",
    "s3_uri": "s3://igvf-files/2024/10/15/bbf9d645-2a44-47c3-bcca-19051579cca2/IGVFFI1153QAAC.bam",
    "schema_version": "11",
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/pSM44-High-R3_S12.sorted.bam",
    "summary": "custom assembly unfiltered alignments",
    "upload_status": "validated",
    "uuid": "bbf9d645-2a44-47c3-bcca-19051579cca2"
}