IGVFFI0821IMHD

released

validated

File Set

IGVFDS5218AVPW(CRISPR activation FACS screen targeting IL2RA: alignments)

Summary

custom assembly unfiltered alignments

Analysis Step Version

/analysis-step-versions/f9f6ae88-d515-4e49-9902-deea3cf87703/

File Format

bam

Content Type

alignments

Content Summary

unfiltered alignments

Aliases

charles-gersbach:jurkats-alignment-crispra-vp64x2-il2ra-high-bin-rep1

md5sum

a64c18f19f87d968ef2fa97d68e6fbd0

Content MD5sum

fc3516de38730a233fafb6024148a54e

File Size

17 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/pSM26-High-R1_S2.sorted.bam

Alignment Details

Reference Files

IGVFFI5804OZJN

Samples

6 items

Sample Terms

Summary

IGVFSM1838CFFU

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM2429HKWG

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM3244YHOI

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM4817IZZH

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM5112IFQB

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

IGVFSM6964ODEE

InVitroSystem

Jurkat

Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)