{
"@context": "/terms/",
"@id": "/alignment-files/IGVFFI0253FOKN/",
"@type": [
"AlignmentFile",
"File",
"Item"
],
"accession": "IGVFFI0253FOKN",
"aliases": [
"charles-gersbach:cd8-tcells-rna-seq-cas-ko-gata3-bam-rep1"
],
"analysis_step_version": "/analysis-step-versions/7298947c-9675-4de2-933a-642bde562751/",
"assay_titles": [
"RNA-seq"
],
"assembly": "GRCh38",
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "e12fd0623fb89e84eb3dfdf8edc6b20d",
"content_summary": "filtered transcriptome alignments",
"content_type": "transcriptome alignments",
"controlled_access": false,
"creation_timestamp": "2024-10-14T21:03:11.973311+00:00",
"derived_from": [
"/sequence-files/IGVFFI0104SKIL/",
"/sequence-files/IGVFFI5972LOIZ/"
],
"file_format": "bam",
"file_set": {
"@id": "/analysis-sets/IGVFDS0006MFFD/",
"accession": "IGVFDS0006MFFD",
"file_set_type": "intermediate analysis",
"samples": [
{
"@id": "/primary-cells/IGVFSM9627TJZO/",
"accession": "IGVFSM9627TJZO",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM0829UPAR/",
"accession": "IGVFSM0829UPAR",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7117ATUZ/",
"accession": "IGVFSM7117ATUZ",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"summary": "knockout RNA-seq: transcriptome alignments"
},
"file_size": 2225103137,
"filtered": true,
"gene_list_for": [],
"href": "/alignment-files/IGVFFI0253FOKN/@@download/IGVFFI0253FOKN.bam",
"input_file_for": [
"/tabular-files/IGVFFI2071KQLY/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "42059e26959146c772f2e2c734e6a6bc",
"read_count": 51946412,
"redacted": false,
"reference_files": [
"/reference-files/IGVFFI0653VCGH/"
],
"release_timestamp": "2024-10-25T04:18:36.462947+00:00",
"s3_uri": "s3://igvf-files/2024/10/14/61d83957-f984-4730-a672-0909c61c691f/IGVFFI0253FOKN.bam",
"schema_version": "11",
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/cd8.rnaseq.pSM73.gata3.d1.star2.Aligned.out.sorted.bam",
"summary": "GRCh38 filtered transcriptome alignments",
"upload_status": "validated",
"uuid": "61d83957-f984-4730-a672-0909c61c691f"
}