CRISPR knockout FACS screen targeting IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes with low FACS signal control
Charles Gersbach, Duke
Samples
Homo sapiens CD8-positive, alpha-beta memory T primary cell, modified with CRISPRko Sp-Cas9, transfected with a expression vector library, guide library, sorted on expression of IL7R