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Ansuman Satpathy

Single-cell Mapping Center for Human Regulatory Elements and Gene Activity

Ansuman Satpathy, Stanford

10x multiome with MULTI-seq

multiplexed sample: Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours; K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB; ... and 8 more samples

Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours

Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

interference single-nucleus ATAC-seq (10x multiome with MULTI-seq)

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