IGVFFI8884FZOC

released
validated
{
    "@context": "/terms/",
    "@id": "/reference-files/IGVFFI8884FZOC/",
    "@type": [
        "ReferenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI8884FZOC",
    "aliases": [
        "charles-gersbach:cd8-tcells-CD2-crispr-screen-grnas-fasta"
    ],
    "assay_titles": [],
    "assembly": "custom",
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released reference file [IGVFFI8884FZOC](/reference-files/IGVFFI8884FZOC/) has in progress subobject curated set [IGVFDS1006FHCX](/curated-sets/IGVFDS1006FHCX/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/reference-files/IGVFFI8884FZOC/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "3d99014485330f031296d35600b258c9",
    "content_type": "elements reference",
    "controlled_access": false,
    "creation_timestamp": "2024-10-15T18:31:54.562017+00:00",
    "external": false,
    "file_format": "fasta",
    "file_set": {
        "@id": "/curated-sets/IGVFDS1006FHCX/",
        "accession": "IGVFDS1006FHCX",
        "file_set_type": "guide RNAs",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM4694MBIW/",
                "accession": "IGVFSM4694MBIW",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM6350DJQS/",
                "accession": "IGVFSM6350DJQS",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM7658WDNM/",
                "accession": "IGVFSM7658WDNM",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM7030KVXG/",
                "accession": "IGVFSM7030KVXG",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM1730FNFX/",
                "accession": "IGVFSM1730FNFX",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM3002NRDK/",
                "accession": "IGVFSM3002NRDK",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "in progress",
        "summary": "Homo sapiens GRCh38 custom guide RNAs",
        "taxa": "Homo sapiens"
    },
    "file_size": 3798,
    "gene_list_for": [],
    "href": "/reference-files/IGVFFI8884FZOC/@@download/IGVFFI8884FZOC.fasta.gz",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "07cbdcac7f40c491ee395c92410ff716",
    "release_timestamp": "2024-12-16T18:54:58.168261+00:00",
    "s3_uri": "s3://igvf-files/2024/10/15/d445a81b-3887-4bc8-abb6-23a8d5e3fd92/IGVFFI8884FZOC.fasta.gz",
    "schema_version": "15",
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CRISPRi.CD2.grna.fa.gz",
    "summary": "custom assembly elements reference",
    "upload_status": "validated",
    "uuid": "d445a81b-3887-4bc8-abb6-23a8d5e3fd92"
}