{
"@context": "/terms/",
"@id": "/reference-files/IGVFFI6999YLZG/",
"@type": [
"ReferenceFile",
"File",
"Item"
],
"accession": "IGVFFI6999YLZG",
"aliases": [
"charles-gersbach:cd8-tcells-TF-CCR7-crispr-screen-grnas-fasta"
],
"assay_titles": [],
"assembly": "custom",
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released reference file [IGVFFI6999YLZG](/reference-files/IGVFFI6999YLZG/) has in progress subobject curated set [IGVFDS3913KUFQ](/curated-sets/IGVFDS3913KUFQ/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/reference-files/IGVFFI6999YLZG/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "18237f63c127cad5d096a0188ac3239b",
"content_type": "elements reference",
"controlled_access": false,
"creation_timestamp": "2024-10-15T18:32:35.840218+00:00",
"external": false,
"file_format": "fasta",
"file_set": {
"@id": "/curated-sets/IGVFDS3913KUFQ/",
"accession": "IGVFDS3913KUFQ",
"file_set_type": "guide RNAs",
"samples": [
{
"@id": "/primary-cells/IGVFSM5048YTHB/",
"accession": "IGVFSM5048YTHB",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM5707IHVB/",
"accession": "IGVFSM5707IHVB",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7413JCSK/",
"accession": "IGVFSM7413JCSK",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM9398LTWW/",
"accession": "IGVFSM9398LTWW",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM2207SJEN/",
"accession": "IGVFSM2207SJEN",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM1205VMNO/",
"accession": "IGVFSM1205VMNO",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM1343OUDU/",
"accession": "IGVFSM1343OUDU",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM1108NFPI/",
"accession": "IGVFSM1108NFPI",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7890DZST/",
"accession": "IGVFSM7890DZST",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7418TTPB/",
"accession": "IGVFSM7418TTPB",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM6002TGBI/",
"accession": "IGVFSM6002TGBI",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM4110AXZO/",
"accession": "IGVFSM4110AXZO",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM8964QQSX/",
"accession": "IGVFSM8964QQSX",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7634EVUP/",
"accession": "IGVFSM7634EVUP",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM1721KPGX/",
"accession": "IGVFSM1721KPGX",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"summary": "Homo sapiens GRCh38 custom guide RNAs",
"taxa": "Homo sapiens"
},
"file_size": 20722,
"gene_list_for": [],
"href": "/reference-files/IGVFFI6999YLZG/@@download/IGVFFI6999YLZG.fasta.gz",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "1dbc8c923e15f765efe6494b6a364f91",
"release_timestamp": "2024-11-18T21:33:15.837734+00:00",
"s3_uri": "s3://igvf-files/2024/10/15/0977d9e4-7eae-4e8a-a683-bb59d4b9542b/IGVFFI6999YLZG.fasta.gz",
"schema_version": "15",
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CRISPR.TF.CCR7.grna.fa.gz",
"summary": "custom assembly elements reference",
"upload_status": "validated",
"uuid": "0977d9e4-7eae-4e8a-a683-bb59d4b9542b"
}