{
"@context": "/terms/",
"@id": "/reference-files/IGVFFI5804OZJN/",
"@type": [
"ReferenceFile",
"File",
"Item"
],
"accession": "IGVFFI5804OZJN",
"aliases": [
"charles-gersbach:cd8-tcells-IL2RA-sa-crispr-screen-grnas-fasta"
],
"assay_titles": [],
"assembly": "custom",
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released reference file [IGVFFI5804OZJN](/reference-files/IGVFFI5804OZJN/) has in progress subobject curated set [IGVFDS3467ONBF](/curated-sets/IGVFDS3467ONBF/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/reference-files/IGVFFI5804OZJN/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "0346c022c48a97f7165b2fee37f87a1f",
"content_type": "elements reference",
"controlled_access": false,
"creation_timestamp": "2024-10-15T18:32:15.126695+00:00",
"external": false,
"file_format": "fasta",
"file_set": {
"@id": "/curated-sets/IGVFDS3467ONBF/",
"accession": "IGVFDS3467ONBF",
"file_set_type": "guide RNAs",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM6490XCFZ/",
"accession": "IGVFSM6490XCFZ",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM6373RONE/",
"accession": "IGVFSM6373RONE",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM8744PJOL/",
"accession": "IGVFSM8744PJOL",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM6178KGGZ/",
"accession": "IGVFSM6178KGGZ",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM6721RTTV/",
"accession": "IGVFSM6721RTTV",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM6606RDWT/",
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"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM1480DJBL/",
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"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM4401YRWQ/",
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"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM3606VJWU/",
"accession": "IGVFSM3606VJWU",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM1838CFFU/",
"accession": "IGVFSM1838CFFU",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM2429HKWG/",
"accession": "IGVFSM2429HKWG",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM5112IFQB/",
"accession": "IGVFSM5112IFQB",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM3148FRBY/",
"accession": "IGVFSM3148FRBY",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM2737ATVK/",
"accession": "IGVFSM2737ATVK",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM7748IVLZ/",
"accession": "IGVFSM7748IVLZ",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-VP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM4817IZZH/",
"accession": "IGVFSM4817IZZH",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM6964ODEE/",
"accession": "IGVFSM6964ODEE",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/in-vitro-systems/IGVFSM3244YHOI/",
"accession": "IGVFSM3244YHOI",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"modality": "activation",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"summary": "Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"summary": "Homo sapiens GRCh38 custom guide RNAs",
"taxa": "Homo sapiens"
},
"file_size": 3803,
"gene_list_for": [],
"href": "/reference-files/IGVFFI5804OZJN/@@download/IGVFFI5804OZJN.fasta.gz",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "07cc3ebf1bf3436b75994ab7b6e5c96b",
"release_timestamp": "2024-11-18T21:29:46.252456+00:00",
"s3_uri": "s3://igvf-files/2024/10/15/8ffcee09-9551-4d6c-b9a2-6bbdd96380f6/IGVFFI5804OZJN.fasta.gz",
"schema_version": "15",
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CRISPR.IL2RA_Sa_Lib.fa.gz",
"summary": "custom assembly elements reference",
"upload_status": "validated",
"uuid": "8ffcee09-9551-4d6c-b9a2-6bbdd96380f6"
}