IGVFFI0626PVXM

released
validated
{
    "@context": "/terms/",
    "@id": "/reference-files/IGVFFI0626PVXM/",
    "@type": [
        "ReferenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI0626PVXM",
    "aliases": [
        "charles-gersbach:cd8-tcells-TFome-Brunello-crispr-screen-grnas-fasta"
    ],
    "assay_titles": [],
    "assembly": "custom",
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released reference file [IGVFFI0626PVXM](/reference-files/IGVFFI0626PVXM/) has in progress subobject curated set [IGVFDS8695PIUQ](/curated-sets/IGVFDS8695PIUQ/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/reference-files/IGVFFI0626PVXM/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "c8e8aca322d3cee71e49b84a50fbca2a",
    "content_type": "elements reference",
    "controlled_access": false,
    "creation_timestamp": "2024-10-15T18:32:56.312381+00:00",
    "external": false,
    "file_format": "fasta",
    "file_set": {
        "@id": "/curated-sets/IGVFDS8695PIUQ/",
        "accession": "IGVFDS8695PIUQ",
        "file_set_type": "guide RNAs",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM9974ALBF/",
                "accession": "IGVFSM9974ALBF",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM3910UHIY/",
                "accession": "IGVFSM3910UHIY",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM2824DRGN/",
                "accession": "IGVFSM2824DRGN",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM2082DWDD/",
                "accession": "IGVFSM2082DWDD",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM4936HKEW/",
                "accession": "IGVFSM4936HKEW",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM2624KTRK/",
                "accession": "IGVFSM2624KTRK",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM3895SURE/",
                "accession": "IGVFSM3895SURE",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM7158ADKU/",
                "accession": "IGVFSM7158ADKU",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM0466RHGQ/",
                "accession": "IGVFSM0466RHGQ",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM7226CVDS/",
                "accession": "IGVFSM7226CVDS",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM8084QKPW/",
                "accession": "IGVFSM8084QKPW",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM7887TFLH/",
                "accession": "IGVFSM7887TFLH",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
                        "modality": "knockout",
                        "status": "released",
                        "summary": "CRISPRko Sp-Cas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "status": "released",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "status": "released",
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "in progress",
        "summary": "Homo sapiens GRCh38 custom guide RNAs",
        "taxa": "Homo sapiens"
    },
    "file_size": 66529,
    "gene_list_for": [],
    "href": "/reference-files/IGVFFI0626PVXM/@@download/IGVFFI0626PVXM.fasta.gz",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "875fcd15ca412d851c3674ba4b68d330",
    "release_timestamp": "2024-12-16T18:54:57.373014+00:00",
    "s3_uri": "s3://igvf-files/2024/10/15/0e128555-f0ba-4bd7-bd39-553b382d9939/IGVFFI0626PVXM.fasta.gz",
    "schema_version": "15",
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CRISPR.TFome.ko.brunello.grna.fa.gz",
    "summary": "custom assembly elements reference",
    "upload_status": "validated",
    "uuid": "0e128555-f0ba-4bd7-bd39-553b382d9939"
}