{
"@context": "/terms/",
"@id": "/reference-files/IGVFFI0626PVXM/",
"@type": [
"ReferenceFile",
"File",
"Item"
],
"accession": "IGVFFI0626PVXM",
"aliases": [
"charles-gersbach:cd8-tcells-TFome-Brunello-crispr-screen-grnas-fasta"
],
"assay_titles": [],
"assembly": "custom",
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released reference file [IGVFFI0626PVXM](/reference-files/IGVFFI0626PVXM/) has in progress subobject curated set [IGVFDS8695PIUQ](/curated-sets/IGVFDS8695PIUQ/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/reference-files/IGVFFI0626PVXM/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "c8e8aca322d3cee71e49b84a50fbca2a",
"content_type": "elements reference",
"controlled_access": false,
"creation_timestamp": "2024-10-15T18:32:56.312381+00:00",
"external": false,
"file_format": "fasta",
"file_set": {
"@id": "/curated-sets/IGVFDS8695PIUQ/",
"accession": "IGVFDS8695PIUQ",
"file_set_type": "guide RNAs",
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{
"@id": "/primary-cells/IGVFSM9974ALBF/",
"accession": "IGVFSM9974ALBF",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM3910UHIY/",
"accession": "IGVFSM3910UHIY",
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"primary cell"
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
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"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
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"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM2824DRGN/",
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"primary cell"
],
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
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"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM2082DWDD/",
"accession": "IGVFSM2082DWDD",
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"primary cell"
],
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
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"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM4936HKEW/",
"accession": "IGVFSM4936HKEW",
"classifications": [
"primary cell"
],
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
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"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM2624KTRK/",
"accession": "IGVFSM2624KTRK",
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"primary cell"
],
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM3895SURE/",
"accession": "IGVFSM3895SURE",
"classifications": [
"primary cell"
],
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7158ADKU/",
"accession": "IGVFSM7158ADKU",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM0466RHGQ/",
"accession": "IGVFSM0466RHGQ",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7226CVDS/",
"accession": "IGVFSM7226CVDS",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
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"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM8084QKPW/",
"accession": "IGVFSM8084QKPW",
"classifications": [
"primary cell"
],
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
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"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
},
{
"@id": "/primary-cells/IGVFSM7887TFLH/",
"accession": "IGVFSM7887TFLH",
"classifications": [
"primary cell"
],
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{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"status": "in progress",
"summary": "Homo sapiens GRCh38 custom guide RNAs",
"taxa": "Homo sapiens"
},
"file_size": 66529,
"gene_list_for": [],
"href": "/reference-files/IGVFFI0626PVXM/@@download/IGVFFI0626PVXM.fasta.gz",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"loci_list_for": [],
"md5sum": "875fcd15ca412d851c3674ba4b68d330",
"release_timestamp": "2024-12-16T18:54:57.373014+00:00",
"s3_uri": "s3://igvf-files/2024/10/15/0e128555-f0ba-4bd7-bd39-553b382d9939/IGVFFI0626PVXM.fasta.gz",
"schema_version": "15",
"status": "released",
"submitted_by": {
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"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/reference_data/AIM_2_T_cell_project_1/CRISPR.TFome.ko.brunello.grna.fa.gz",
"summary": "custom assembly elements reference",
"upload_status": "validated",
"uuid": "0e128555-f0ba-4bd7-bd39-553b382d9939"
}