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Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements

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Title
Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements
Authors
Florence M. Chardon, Troy A. McDiarmid, Nicholas F. Page, Beth Martin, Silvia Domcke, Samuel G. Regalado, Jean-Benoît Lalanne, Diego Calderon, Lea M. Starita, Stephan J. Sanders, Nadav Ahituv, Jay Shendure
Citation
Florence M. Chardon, Troy A. McDiarmid, Nicholas F. Page, Beth Martin, Silvia Domcke, Samuel G. Regalado, Jean-Benoît Lalanne, Diego Calderon, Lea M. Starita, Stephan J. Sanders, Nadav Ahituv, Jay Shendure (2023, March 28). Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements. bioRxiv.  
Publication Identifiers
Attribution
Jay Shendure, UW

Samples

17 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

File Sets

68 items
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_neurons_HMGA1_rep1
Jay Shendure, UW
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_K562_DNMT3B_rep1
Jay Shendure, UW
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_K562_DNMT3B_rep2
Jay Shendure, UW
MeasurementSet
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane2_exprs
Jay Shendure, UW
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_K562_TBR1_rep1
Jay Shendure, UW
MeasurementSet
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_exprs
Jay Shendure, UW
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_K562_ANXA1_rep2
Jay Shendure, UW
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_K562_HMGA1_rep2
Jay Shendure, UW
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_K562_FOXP1_rep3
Jay Shendure, UW
MeasurementSet
CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlevalidations_K562_TCF4_rep1
Jay Shendure, UW