HG011966

Ian Whaling

Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems

Jay Shendure

jay-shendure

Jay Shendure, UW

Nadav Ahituv

Martin Kircher

Guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane2), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane1), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

scCRISPR activation screen: differential gene expression quantifications, sparse gene count matrix

K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements

K562 cell line, female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days, male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

K562 cell line, female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements

Samples

File Sets

Attribution