Home

Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements

released
Title
Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements
Authors
Florence M. Chardon, Troy A. McDiarmid, Nicholas F. Page, Beth Martin, Silvia Domcke, Samuel G. Regalado, Jean-Benoît Lalanne, Diego Calderon, Lea M. Starita, Stephan J. Sanders, Nadav Ahituv, Jay Shendure
Citation
Florence M. Chardon, Troy A. McDiarmid, Nicholas F. Page, Beth Martin, Silvia Domcke, Samuel G. Regalado, Jean-Benoît Lalanne, Diego Calderon, Lea M. Starita, Stephan J. Sanders, Nadav Ahituv, Jay Shendure (2023, March 28). Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements. bioRxiv.  
Publication Identifiers

Samples

11 items
Sample Terms
Summary
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with a guide library
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with a guide library
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane1), male, Homo sapiens modified with a guide library
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3), male, Homo sapiens modified with a guide library
InVitroSystem
K562 cell line, female, Homo sapiens modified with a guide library
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4), male, Homo sapiens modified with a guide library
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days, male, Homo sapiens modified with a guide library
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with a guide library
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with a guide library
InVitroSystem
K562 cell line, female, Homo sapiens modified with a guide library

File Sets

19 items
MeasurementSet
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane2_exprs
Jay Shendure, UW
MeasurementSet
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_exprs
Jay Shendure, UW
MeasurementSet
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane4_exprs
Jay Shendure, UW
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane2_CS1
Jay Shendure, UW
MeasurementSet
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_exprs
Jay Shendure, UW
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane3_CS1
Jay Shendure, UW
AnalysisSet
principal analysis of scCRISPR screen data
  • jay-shendure:analysis_set_CRISPRa_ipscNeurons
Jay Shendure, UW
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane1_CS1
Jay Shendure, UW
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane1_CS1
Jay Shendure, UW
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_CS1
Jay Shendure, UW

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW