Construct Library Set

Title
Construct Library Set
Description
Construct library set is a file set that hosts raw data files (e.g. FASTQs) resulting from sequencing of a library prior to its delivery into the samples being investigated. For example sequencing results of guide RNAs after cloning them but prior to their delivery to the actual samples under investigation. The results thus represent the sequencing output of the guides that are then introduced into cells, but may not always correspond to what exact guides ended up being delivered or expressed. Not all construct library sets will end up having FASTQs or any other files in them. For example if the lab chooses not to sequence their guide library prior to delivery, no FASTQs will be required in that case. Construct library sets should not be associated with any samples because they are designed to capture the library prior to its delivery and hence, has no relation to the sample that will get the plasmids eventually.
Required
award, file_set_type, lab, scope, selection_criteria
Identifying Properties
accession, aliases, alternate_accessions, uuid
array[string]
Lab specific identifiers to reference an object.
array[linkto]
Ontological terms for diseases or phenotypes associated with the sequence material cloned in this construct library.
number
The average number of guides targeting each element of interest in the library.
number
The average size of the inserts cloned into vectors in the library.
Grant associated with the submission.
array[linkto]
File sets that can serve as scientific controls for this file set.
string
The type of control this file set represents.
string
A plain text description of the object.
array[linkto]
Documents that provide additional information (not data file).
string
An identifier in plain text for the specific exon in an expression vector library. The associated gene must be listed in the small_scale_gene_list property.
string
The type or category of this construct library set.
string
The design of guides used in a CRISPR library, paired-guide (pgRNA) or single-guide (sgRNA).
array[linkto]
The files containing sequence material of interest either used for insert design or directly cloned into vectors in this library.
Lab associated with the submission.
The large scale list of (>100 genes) this construct library was designed to target.
A large scale list (>100) of specific chromosomal regions.
string
The lot identifier provided by the originating lab or vendor.
integer
Lower bound of the number of guides targeting each element of interest in the library.
integer
Lower bound of the size of the inserts cloned in vectors in the library.
array[linkto]
List of Open Reading Frame this construct library was designed to target.
string
The product or catalog identifier provided following deposition to addgene.org.
array[linkto]
The publications associated with this object.
string
The scope or scale that this construct library is designed to target.
array[string]
The criteria used to select the sequence material cloned into the library.
array[linkto]
The specific, small scale list of (<=100) gene(s) this construct library was designed to target. This property differs from targeted_genes in Measurement Set, which describes genes targeted for binding sites or used for sorting by expression.
array[object]
A small scale (<=100) list of specific chromosomal region(s).
array[linkto]
The originating lab(s) or vendor(s).
string
Additional information specified by the submitter to be displayed as a comment on the portal.
string
An identifier in plain text for the specific targeton in an editing template library. The associated gene must be listed in the small_scale_gene_list property.
object
The coordinates and an identifier in plain text for the specific tile of a protein region cloned in an expression vector library. The associated gene for this protein must be listed in the small_scale_gene_list or large_scale_gene_list property.
string
The tiling modality of guides across elements or loci in a CRISPR library.
integer
Upper bound of the number of guides targeting each element of interest in the library.
integer
Upper bound of the size of the inserts cloned in vectors in the library.