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CD8-positive, alpha-beta memory T cell — IGVFSM9974ALBF

released
Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Taxa
Homo sapiens
Sources
stem-cell-technologies
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispr-ko-tfome-rep1
Publications

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-23
unspecified
Homo sapiens

File Sets

1 item
Accession
Summary
Aliases
Lab
Homo sapiens GRCh38 custom guide RNAs
  • charles-gersbach:tfome-grnas-set
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
knockout

Sorted Fractions of Sample

2 items
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke