HG012053

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Lentivirus encoding for CRISPRa (VP64-dSaCas9-VP64) and a TF-targeting gRNA library

gRNA sequencing for activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

activation Perturb-seq: filtered feature barcode matrix

activation Perturb-seq: differential gene expression quantifications

CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)