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CD8-positive, alpha-beta memory T cell — IGVFSM8036VPRU

released
Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Taxa
Homo sapiens
Description
Lentivirus encoding for CRISPRa (VP64-dSaCas9-VP64) and a TF-targeting gRNA library
Sources
stem-cell-technologies
Submitter Comment
The ConstructLibrarySet on this Sample was upgraded from a ConstructLibrary attached to the FileSet it was connected to.
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispra-rep1
Publications

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-6
unspecified
Homo sapiens

File Sets

5 items
activation Perturb-seq: filtered feature barcode matrix
  • charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispra
Charles Gersbach, Duke
Homo sapiens GRCh38 guide RNAs
  • charles-gersbach:cd8-tcells-perturb-seq-grnas-set
Charles Gersbach, Duke
activation Perturb-seq: differential gene expression quantifications
  • charles-gersbach:cd8-tcells-analysis-set-perturb-seq-crispra
Charles Gersbach, Duke
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep1
Charles Gersbach, Duke
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep1
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
activation

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke