CD8-positive, alpha-beta memory T cell, Homo sapiens transduced (lentivirus) with a expression vector library
CD8 T cells were cultured in PRIME-XV T cell expansion XSFM (FujiFilm) supplemented with 5% human platelet lysate (Compass Biomed), 100 units/mL of penicilllin, 100 units/mL of streptomycin, and 100 units/mL human IL-2 (Peprotech).
Human CD8+ T cells were transduced with lentivirus encoding for either HER2-CAR-2A-GFP or HER2-CAR-2A-BATF3.
T cells were stimulated repeatedly every three days with a 3:1 CD3/CD28 dynabead to cell ratio (chronic stimulation).
50,000 transduced CD8 T cells were sorted for ATAC-seq on day 14.
ATAC-seq libraries were prepared according to the Omni ATAC-seq protocol.
charles-gersbach:cd8-tcells-primary-cell-chronic-activation-batf3-rep2