HG012053

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach

charles-gersbach

Charles Gersbach, Duke

Gregory Crawford

Tim Reddy

CD8-positive, alpha-beta memory T cell — IGVFSM7226CVDS

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CRISPR FACS screen

CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

CRISPR knockout FACS screen targeting IL7R: alignments

CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications

STEMCELL Technologies

Alejandro Barrera

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell — IGVFSM7226CVDS

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