{
"@context": "/terms/",
"@id": "/primary-cells/IGVFSM6449HHZS/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6449HHZS",
"age": "unknown",
"aliases": [
"charles-gersbach:hPA-primary-cell-perturbseq-10xlane2"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released primary cell [IGVFSM6449HHZS](/primary-cells/IGVFSM6449HHZS/) has in progress subobject treatment [e23aab02-ae82-4935-85ad-1f238757f3ba](/treatments/e23aab02-ae82-4935-85ad-1f238757f3ba/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/primary-cells/IGVFSM6449HHZS/"
},
{
"category": "mismatched status",
"detail": "Released primary cell [IGVFSM6449HHZS](/primary-cells/IGVFSM6449HHZS/) has in progress subobject treatment [c7e73583-b639-473f-84c4-947cdc0bd81d](/treatments/c7e73583-b639-473f-84c4-947cdc0bd81d/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/primary-cells/IGVFSM6449HHZS/"
},
{
"category": "missing publication",
"detail": "Primary cell [IGVFSM6449HHZS](/primary-cells/IGVFSM6449HHZS/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/primary-cells/IGVFSM6449HHZS/"
},
{
"category": "missing publication",
"detail": "Construct library set [IGVFDS6102AFVS](/construct-library-sets/IGVFDS6102AFVS/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/construct-library-sets/IGVFDS6102AFVS/"
},
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS6102AFVS](/construct-library-sets/IGVFDS6102AFVS/) has in progress subobject reference file [IGVFFI4279ZUTM](/reference-files/IGVFFI4279ZUTM/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS6102AFVS/"
}
],
"WARNING": [
{
"category": "missing age",
"detail": "Primary cell [IGVFSM6449HHZS](/primary-cells/IGVFSM6449HHZS/) is missing `upper_bound_age`, `lower_bound_age`, and `age_units`. Tissues, primary cells, and whole organisms are expected to specify a lower bound age, upper bound age, and age units.",
"level": 40,
"level_name": "WARNING",
"name": "audit_biosample_age",
"path": "/primary-cells/IGVFSM6449HHZS/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"classifications": [
"primary cell"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6102AFVS/",
"accession": "IGVFDS6102AFVS",
"file_set_type": "guide library",
"status": "released"
}
],
"creation_timestamp": "2025-03-28T16:58:07.157992+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO6921DDPB/",
"accession": "IGVFDO6921DDPB",
"status": "released"
}
],
"embryonic": false,
"file_sets": [
{
"@id": "/auxiliary-sets/IGVFDS1645YFQD/",
"accession": "IGVFDS1645YFQD",
"aliases": [
"charles-gersbach:hPA-auxiliary-set-perturbseq-10xlane2-CRISPR"
],
"file_set_type": "gRNA sequencing",
"lab": {
"title": "Charles Gersbach, Duke"
},
"status": "released",
"summary": "gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes"
},
{
"@id": "/measurement-sets/IGVFDS8404CSTN/",
"accession": "IGVFDS8404CSTN",
"aliases": [
"charles-gersbach:hPA-measurement-set-perturbseq-10xlane2-GEX"
],
"file_set_type": "experimental data",
"lab": {
"title": "Charles Gersbach, Duke"
},
"preferred_assay_title": "Perturb-seq",
"status": "released",
"summary": "CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"modifications": [
{
"@id": "/crispr-modifications/703c3934-6c9b-41d0-8481-e73cc64075f7/",
"cas": "dCas9",
"cas_species": "Streptococcus pyogenes (Sp)",
"fused_domain": "2xVP64-2A-Puro",
"modality": "activation",
"status": "released",
"summary": "CRISPRa Sp-dCas9-2xVP64-2A-Puro"
}
],
"multiplexed_in": [],
"nucleic_acid_delivery": "lentiviral transduction",
"origin_of": [],
"parts": [],
"pooled_in": [],
"protocols": [
"https://www.protocols.io/view/human-primary-astrocyte-hpa-culture-x54v97jwzg3e/v1",
"https://www.protocols.io/view/human-primary-astrocyte-hpa-crispra-reprogramming-ewov12477gr2/v1"
],
"release_timestamp": "2025-05-16T16:07:10.475983+00:00",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000127/",
"status": "released",
"term_name": "astrocyte"
}
],
"schema_version": "23",
"sex": "unspecified",
"sorted_fractions": [],
"sources": [
{
"@id": "/sources/sciencell/",
"status": "released",
"title": "ScienCell"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/3782ce69-0e9f-4b46-91f9-4578139785c7/",
"title": "Ruhi Rai"
},
"summary": "Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/c7e73583-b639-473f-84c4-947cdc0bd81d/",
"depletion": false,
"purpose": "differentiation",
"status": "in progress",
"treatment_term_name": "Brain-Derived Neurotrophic Factor",
"treatment_type": "protein"
},
{
"@id": "/treatments/e23aab02-ae82-4935-85ad-1f238757f3ba/",
"depletion": false,
"purpose": "differentiation",
"status": "in progress",
"treatment_term_name": "Neurotrophin-3",
"treatment_type": "protein"
}
],
"uuid": "1195a792-0684-4c38-8035-38e683e82f9e",
"virtual": false
}