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CD8-positive, alpha-beta memory T cell — IGVFSM5189UBOI

released
Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Taxa
Homo sapiens
Description
Lentivirus encoding for CRISPRi (dSaCas9-KRAB) and a TF-targeting gRNA library
Sources
stem-cell-technologies
Submitter Comment
The ConstructLibrarySet on this Sample was upgraded from a ConstructLibrary attached to the FileSet it was connected to.
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispri-rep3
Publications

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-8
unspecified
Homo sapiens

File Sets

5 items
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep3
Charles Gersbach, Duke
interference Perturb-seq: filtered feature barcode matrix
  • charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispri
Charles Gersbach, Duke
Homo sapiens GRCh38 guide RNAs
  • charles-gersbach:cd8-tcells-perturb-seq-grnas-set
Charles Gersbach, Duke
interference Perturb-seq: differential gene expression quantifications
  • charles-gersbach:cd8-tcells-analysis-set-perturb-seq-crispri
Charles Gersbach, Duke
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep3
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
interference

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke