CD8-positive, alpha-beta memory T cell — IGVFSM4633QTQY

released
Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens transduced (lentivirus) with a expression vector library
Sample Terms
Sex
unspecified
Age
unknown
Taxa
Homo sapiens
Description
Lentivirus encoding for HER2-CAR-2A-GFP or HER2-CAR-2A-BATF3
Sources
Submitter Comment
CD8 T cells were cultured in PRIME-XV T cell expansion XSFM (FujiFilm) supplemented with 5% human platelet lysate (Compass Biomed), 100 units/mL of penicilllin, 100 units/mL of streptomycin, and 100 units/mL human IL-2 (Peprotech). Human CD8+ T cells were transduced with lentivirus encoding for either HER2-CAR-2A-GFP or HER2-CAR-2A-BATF3. T cells were stimulated repeatedly every three days with a 3:1 CD3/CD28 dynabead to cell ratio (chronic stimulation). 50,000 transduced CD8 T cells were sorted for ATAC-seq on day 14. ATAC-seq libraries were prepared according to the Omni ATAC-seq protocol.
Aliases
charles-gersbach:cd8-tcells-primary-cell-chronic-activation-gfp-rep3
Publications
Institutional Certificates

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-11
unspecified
Homo sapiens

File Sets

3 items
RNA-seq: transcriptome alignments
  • charles-gersbach:cd8-tcells-intermediate-analysis-rna-seq-batf3-overexpression-gfp
Charles Gersbach, Duke
RNA-seq: fold change over control, transcript quantifications
  • charles-gersbach:cd8-tcells-analysis-set-rna-seq-batf3-overexpression
Charles Gersbach, Duke
RNA-seq integrating a control transduction expression vector library
  • charles-gersbach:cd8-tcells-rna-seq-chronic-activation-gfp-rep3
Charles Gersbach, Duke

Attribution