CD8-positive, alpha-beta memory T cell — IGVFSM3427LEEW

released
{
    "@context": "/terms/",
    "@id": "/primary-cells/IGVFSM3427LEEW/",
    "@type": [
        "PrimaryCell",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "IGVFSM3427LEEW",
    "age": "unknown",
    "aliases": [
        "charles-gersbach:cd8-tcells-primary-cell-crispri-b2m-low-bin-rep2"
    ],
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released construct library set [IGVFDS5026WRUO](/construct-library-sets/IGVFDS5026WRUO/) has in progress subobject tabular file [IGVFFI1466VTFO](/tabular-files/IGVFFI1466VTFO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/construct-library-sets/IGVFDS5026WRUO/"
            }
        ],
        "WARNING": [
            {
                "category": "missing files",
                "detail": "Construct library set [IGVFDS5026WRUO](/construct-library-sets/IGVFDS5026WRUO/) has no `files`. File sets are expected to have files.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_no_files",
                "path": "/construct-library-sets/IGVFDS5026WRUO/"
            },
            {
                "category": "missing age",
                "detail": "Primary cell [IGVFSM3427LEEW](/primary-cells/IGVFSM3427LEEW/) is missing `upper_bound_age`, `lower_bound_age`, and `age_units`. Tissues, primary cells, and whole organisms are expected to specify a lower bound age, upper bound age, and age units.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_biosample_age",
                "path": "/primary-cells/IGVFSM3427LEEW/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "classifications": [
        "primary cell"
    ],
    "construct_library_sets": [
        {
            "@id": "/construct-library-sets/IGVFDS5026WRUO/",
            "accession": "IGVFDS5026WRUO",
            "file_set_type": "guide library",
            "status": "released"
        }
    ],
    "creation_timestamp": "2023-11-08T19:00:49.545544+00:00",
    "donors": [
        "/human-donors/IGVFDO4200TDAP/"
    ],
    "file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS3559YNWI/",
            "accession": "IGVFDS3559YNWI",
            "aliases": [
                "charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-low-bin-rep2"
            ],
            "file_set_type": "experimental data",
            "lab": {
                "title": "Charles Gersbach, Duke"
            },
            "preferred_assay_title": "CRISPR FACS screen",
            "status": "released",
            "summary": "CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M"
        },
        {
            "@id": "/auxiliary-sets/IGVFDS6947GSPF/",
            "accession": "IGVFDS6947GSPF",
            "aliases": [
                "charles-gersbach:cd8-tcells-auxiliary-set-crispri-b2m-low-bin-rep2"
            ],
            "file_set_type": "gRNA sequencing",
            "lab": {
                "title": "Charles Gersbach, Duke"
            },
            "status": "released",
            "summary": "gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M"
        },
        {
            "@id": "/curated-sets/IGVFDS3778BYII/",
            "accession": "IGVFDS3778BYII",
            "aliases": [
                "charles-gersbach:cd8-tcells-primary-cell-b2m-grnas-set"
            ],
            "file_set_type": "guide RNAs",
            "lab": {
                "title": "Charles Gersbach, Duke"
            },
            "status": "in progress",
            "summary": "Homo sapiens GRCh38 custom guide RNAs"
        },
        {
            "@id": "/analysis-sets/IGVFDS2716FVEL/",
            "accession": "IGVFDS2716FVEL",
            "aliases": [
                "charles-gersbach:cd8-tcells-intermediate-analysis-crispri-b2m"
            ],
            "file_set_type": "intermediate analysis",
            "lab": {
                "title": "Charles Gersbach, Duke"
            },
            "status": "released",
            "summary": "CRISPR interference FACS screen targeting B2M: alignments"
        },
        {
            "@id": "/analysis-sets/IGVFDS8839AFQB/",
            "accession": "IGVFDS8839AFQB",
            "aliases": [
                "charles-gersbach:cd8-tcells-analysis-set-crispri-b2m"
            ],
            "file_set_type": "principal analysis",
            "lab": {
                "title": "Charles Gersbach, Duke"
            },
            "status": "released",
            "summary": "CRISPR interference FACS screen targeting B2M: fold change over control, guide quantifications"
        }
    ],
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "modifications": [
        {
            "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
            "cas": "dCas9",
            "cas_species": "Staphylococcus aureus (Sa)",
            "modality": "interference",
            "status": "released",
            "summary": "CRISPRi Sa-dCas9"
        }
    ],
    "moi": 0.4,
    "multiplexed_in": [],
    "notes": "This sample's modifications originally included Modification /modifications/5d269861-f341-4629-a436-492fb432e132/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_5d269861-f341-4629-a436-492fb432e132.",
    "nucleic_acid_delivery": "lentiviral transduction",
    "origin_of": [],
    "parts": [],
    "pooled_in": [],
    "publications": [
        {
            "@id": "/publications/6cc80a53-4ca1-4bd4-9942-f0f335b7ec20/",
            "publication_identifiers": [
                "PMID:37945901",
                "doi:10.1038/s41588-023-01554-0",
                "PMCID:PMC10703699"
            ],
            "status": "released"
        }
    ],
    "release_timestamp": "2024-11-18T21:09:14.526613+00:00",
    "sample_terms": [
        {
            "@id": "/sample-terms/CL_0000909/",
            "status": "released",
            "term_name": "CD8-positive, alpha-beta memory T cell"
        }
    ],
    "schema_version": "21",
    "sex": "unspecified",
    "sorted_fractions": [],
    "sorted_from": {
        "@id": "/primary-cells/IGVFSM0015KLZS/",
        "accession": "IGVFSM0015KLZS",
        "status": "released"
    },
    "sorted_from_detail": "FACS bin for 0-10% expression of b2m",
    "sources": [
        {
            "@id": "/sources/zen-bioscience/",
            "status": "released",
            "title": "Zen Bioscience"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
    "taxa": "Homo sapiens",
    "uuid": "29c9be4e-d6d9-4337-abfc-218e46d9dd1d",
    "virtual": false
}