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CD8-positive, alpha-beta memory T cell — IGVFSM2207SJEN

released
Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Construct Library Sets
  • IGVFDS4163YHBI guide (sgRNA) library targeting transcription start sites in many genes
Taxa
Homo sapiens
Sources
stem-cell-technologies
Sorted From Sample
IGVFSM5048YTHB FACS bin for 90-100% expression of CCR7
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispri-ccr7-high-bin-rep1
Publications

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-20
unspecified
Homo sapiens

File Sets

4 items
CRISPR interference FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:cd8-tcells-measurement-set-crispri-ccr7-high-bin-rep1
Charles Gersbach, Duke
gRNA sequencing
  • charles-gersbach:cd8-tcells-auxiliary-set-crispri-ccr7-high-bin-rep1
Charles Gersbach, Duke
Homo sapiens GRCh38 custom guide RNAs
  • charles-gersbach:cd8-tcells-primary-cell-ccr7-grnas-set
Charles Gersbach, Duke
CRISPR interference FACS screen targeting CCR7: alignments, fold change over control, guide quantifications
  • charles-gersbach:cd8-tcells-analysis-set-crispri-ccr7
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
Activation
interference

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke