HG012053

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach

charles-gersbach

Charles Gersbach, Duke

Gregory Crawford

Tim Reddy

CD8-positive, alpha-beta memory T cell — IGVFSM1205VMNO

audit_item_status

audit_biosample_age

audit_no_files

CRISPR FACS screen

CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes

CRISPR interference FACS screen targeting CCR7: alignments

CRISPR interference FACS screen targeting CCR7: fold change over control, guide quantifications

STEMCELL Technologies

Alejandro Barrera

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

CD8-positive, alpha-beta memory T cell — IGVFSM1205VMNO

Donors

File Sets

Modifications

Attribution