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CD8-positive, alpha-beta memory T cell — IGVFSM1190UUKW

released
Summary
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Construct Library Sets
  • IGVFDS5026WRUO guide (sgRNA) library targeting transcription start sites in B2M
Taxa
Homo sapiens
Sources
zen-bioscience
Sorted From Sample
IGVFSM2690MTOA FACS bin for 0-10% expression of b2m
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispri-b2m-low-bin-rep3
Publications
Attribution
Charles Gersbach, Duke

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-19
unspecified
Homo sapiens

File Sets

3 items
gRNA sequencing
  • charles-gersbach:cd8-tcells-auxiliary-set-crispri-b2m-low-bin-rep3
Charles Gersbach, Duke
CRISPR interference FACS screen targeting B2M: alignments, fold change over control, guide quantifications
  • charles-gersbach:cd8-tcells-analysis-set-crispri-b2m
Charles Gersbach, Duke
low FACS signal CRISPR interference FACS screen sorted on the expression of B2M integrating a guide (sgRNA) library targeting transcription start sites in B2M
  • charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-low-bin-rep3
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
Activation
interference