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CD8-positive, alpha-beta memory T cell — IGVFSM1108NFPI

released
Summary
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Sex
unspecified
Age
unknown
Construct Library Sets
  • IGVFDS4163YHBI guide (sgRNA) library targeting transcription start sites in many genes
Taxa
Homo sapiens
Sources
stem-cell-technologies
Aliases
charles-gersbach:cd8-tcells-primary-cell-crispra-ccr7-rep2
Publications
Attribution
Charles Gersbach, Duke

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-21
unspecified
Homo sapiens

Modifications

1 item
Summary
Modality
Activation
activation

Sorted Fractions of Sample

2 items
Sample Terms
Summary
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
PrimaryCell
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)