CD8-positive, alpha-beta memory T cell — IGVFSM0066TACG

released
Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens transduced (lentivirus) with a expression vector library
Sample Terms
Sex
unspecified
Age
unknown
Construct Library Sets
Taxa
Homo sapiens
Description
Lentivirus encoding for HER2-CAR-2A-GFP or HER2-CAR-2A-BATF3
Sources
Submitter Comment
CD8 T cells were cultured in PRIME-XV T cell expansion XSFM (FujiFilm) supplemented with 5% human platelet lysate (Compass Biomed), 100 units/mL of penicilllin, 100 units/mL of streptomycin, and 100 units/mL human IL-2 (Peprotech). Human CD8+ T cells were transduced with lentivirus encoding for either HER2-CAR-2A-GFP or HER2-CAR-2A-BATF3. T cells were stimulated once with CD3/CD28 dynabeads (acute stimulation). 50,000 transduced CD8 T cells were sorted for ATAC-seq on day 14. ATAC-seq libraries were prepared according to the Omni ATAC-seq protocol.
Aliases
charles-gersbach:cd8-tcells-primary-cell-acute-activation-batf3-rep1
Publications
Institutional Certificates

Attribution