CD8-positive, alpha-beta memory T cell — IGVFSM0015KLZS

released
1{
2    "@context": "/terms/",
3    "@id": "/primary-cells/IGVFSM0015KLZS/",
4    "@type": [
5        "PrimaryCell",
6        "Biosample",
7        "Sample",
8        "Item"
9    ],
10    "accession": "IGVFSM0015KLZS",
11    "age": "unknown",
12    "aliases": [
13        "charles-gersbach:cd8-tcells-primary-cell-crispri-b2m-rep2"
14    ],
15    "audit": {
16        "INTERNAL_ACTION": [
17            {
18                "category": "missing publication",
19                "detail": "Construct library set [IGVFDS5026WRUO](/construct-library-sets/IGVFDS5026WRUO/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
20                "level": 30,
21                "level_name": "INTERNAL_ACTION",
22                "name": "audit_file_set_missing_publication",
23                "path": "/construct-library-sets/IGVFDS5026WRUO/"
24            }
25        ],
26        "WARNING": [
27            {
28                "category": "missing age",
29                "detail": "Primary cell [IGVFSM0015KLZS](/primary-cells/IGVFSM0015KLZS/) is missing `upper_bound_age`, `lower_bound_age`, and `age_units`. Tissues, primary cells, and whole organisms are expected to specify a lower bound age, upper bound age, and age units.",
30                "level": 40,
31                "level_name": "WARNING",
32                "name": "audit_biosample_age",
33                "path": "/primary-cells/IGVFSM0015KLZS/"
34            }
35        ]
36    },
37    "award": {
38        "@id": "/awards/HG012053/",
39        "component": "functional characterization"
40    },
41    "classifications": [
42        "primary cell"
43    ],
44    "construct_library_sets": [
45        {
46            "@id": "/construct-library-sets/IGVFDS5026WRUO/",
47            "accession": "IGVFDS5026WRUO",
48            "file_set_type": "guide library",
49            "status": "released"
50        }
51    ],
52    "creation_timestamp": "2023-11-08T19:00:42.597532+00:00",
53    "donors": [
54        {
55            "@id": "/human-donors/IGVFDO4200TDAP/",
56            "accession": "IGVFDO4200TDAP",
57            "status": "released"
58        }
59    ],
60    "file_sets": [],
61    "institutional_certificates": [],
62    "lab": {
63        "@id": "/labs/charles-gersbach/",
64        "title": "Charles Gersbach, Duke"
65    },
66    "modifications": [
67        {
68            "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
69            "cas": "dCas9",
70            "cas_species": "Staphylococcus aureus (Sa)",
71            "fused_domain": "KOX1-KRAB",
72            "modality": "interference",
73            "status": "released",
74            "summary": "CRISPRi Sa-dCas9-KOX1-KRAB"
75        }
76    ],
77    "moi": 0.4,
78    "multiplexed_in": [],
79    "notes": "This sample's modifications originally included Modification /modifications/5d269861-f341-4629-a436-492fb432e132/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_5d269861-f341-4629-a436-492fb432e132.",
80    "nucleic_acid_delivery": "lentiviral transduction",
81    "origin_of": [],
82    "parts": [],
83    "pooled_in": [],
84    "publications": [
85        {
86            "@id": "/publications/6cc80a53-4ca1-4bd4-9942-f0f335b7ec20/",
87            "publication_identifiers": [
88                "PMID:37945901",
89                "doi:10.1038/s41588-023-01554-0",
90                "PMCID:PMC10703699"
91            ],
92            "status": "released"
93        }
94    ],
95    "release_timestamp": "2024-11-18T21:09:14.569715+00:00",
96    "sample_terms": [
97        {
98            "@id": "/sample-terms/CL_0000909/",
99            "status": "released",
100            "term_name": "CD8-positive, alpha-beta memory T cell"
101        }
102    ],
103    "schema_version": "22",
104    "sex": "unspecified",
105    "sorted_fractions": [
106        "/primary-cells/IGVFSM4508IIAY/",
107        "/primary-cells/IGVFSM3427LEEW/"
108    ],
109    "sources": [
110        {
111            "@id": "/sources/zen-bioscience/",
112            "status": "released",
113            "title": "Zen Bioscience"
114        }
115    ],
116    "status": "released",
117    "submitted_by": {
118        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
119        "title": "Alejandro Barrera"
120    },
121    "summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)",
122    "taxa": "Homo sapiens",
123    "uuid": "8f49513c-25f8-4058-8874-d7baa540f67c",
124    "virtual": false
125}