in vitro CRISPR screen using flow cytometry

reads

fastq

CRISPR FACS screen

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes

in vitro CRISPR screen using flow cytometry

reads

fastq

CRISPR FACS screen

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating an expression vector library of BATF3 and a guide (sgRNA) library targeting transcription start sites in many genes

in vitro CRISPR screen using flow cytometry

reads

fastq

CRISPR FACS screen

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes and an expression vector library of BATF3

in vitro CRISPR screen using flow cytometry

reads

fastq

CRISPR FACS screen

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)

low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes and an expression vector library of BATF3