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samples.@id
/in-vitro-systems/IGVFSM3086DQMC/
3 items
IGVFDS1298BFAZ
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep2_S56
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep2_S62
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep1_S13
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep2_S68
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep1_S19
  • jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74
in vitro CRISPR screen using flow cytometry
experimental data
CRISPR FlowFISH screen
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
IGVFDS2805FQAD
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep2_S56
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep2_S62
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep1_S13
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep2_S68
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep1_S19
  • jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74
gRNA sequencing
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
revoked
gRNA sequencing
IGVFDS5951VSHM
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF1
cell sorting
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library, HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
released
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets