guide quantifications, fold change over control, alignments

principal analysis

Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4), Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens Jurkat cell line, modified with CRISPRa Sa-dCas9-2xVP64, transfected with a guide library, sorted on expression of IL2RA

CRISPR activation FACS screen targeting IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA with low FACS signal control

in vitro CRISPR screen using flow cytometry

reads

experimental data

CRISPR FACS screen

Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

low FACS signal CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA