Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Homo sapiens CD8-positive, alpha-beta memory T primary cell, modified with CRISPRko Sp-Cas9, transfected with a guide library
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in GATA3 and a non-targeting guide (sgRNA) library with non-targeting control