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THP-1 — IGVFSM0539NUPM

released
Summary
multiplexed sample: Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours; K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB; ... and 8 more samples
Multiplexing Method
barcode based
Sources
Barcode Map
IGVFFI1855MTKY
Taxa
Homo sapiens
Aliases
ansuman-satpathy:multiplexed_sample_igvf10_10x2
Protocols

Donors

4 items
Aliases
  • igvf:donor_of_Jurkat
male
Homo sapiens
  • igvf:donor_of_teloHAEC
female
Homo sapiens
  • igvf:donor_of_THP1
male
Homo sapiens
  • igvf:donor_of_K562
female
Homo sapiens

File Sets

5 items
interference single-nucleus ATAC-seq (10x multiome with MULTI-seq)
  • ansuman-satpathy:snATAC-seq_igvf10_10x2
Ansuman Satpathy, Stanford
10x multiome with MULTI-seq: cell hashing barcodes, fragments, index, peaks, raw feature barcode matrix
  • ansuman-satpathy:igvf10_analysis_set
Ansuman Satpathy, Stanford
10x multiome with MULTI-seq
  • ansuman-satpathy:IGVFDS1013HCXI_IGVFDS3910GBDQ_IGVFDS9139TBCB_IGVFDS2246RAZO_analysis
  • jesse-engreitz:IGVFDS1013HCXI_IGVFDS3910GBDQ_IGVFDS9139TBCB_IGVFDS2246RAZO_analysis
Jesse Engreitz, Stanford
lipid-conjugated oligo sequencing for interference single-nucleus ATAC-seq (10x multiome with MULTI-seq), interference single-nucleus RNA sequencing assay (10x multiome with MULTI-seq)
  • ansuman-satpathy:multiseq_igvf10_10x2
Ansuman Satpathy, Stanford
interference single-nucleus RNA sequencing assay (10x multiome with MULTI-seq)
  • ansuman-satpathy:snRNA-seq_igvf10_10x2
Ansuman Satpathy, Stanford

Multiplexed Samples

10 items
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours
InVitroSystem
K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB
InVitroSystem
Jurkat cell line, male, Homo sapiens
InVitroSystem
THP-1 cell line, male, Homo sapiens
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB
InVitroSystem
Jurkat cell line, male, Homo sapiens
InVitroSystem
Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours
InVitroSystem
K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB
InVitroSystem
THP-1 cell line, male, Homo sapiens

Modifications

1 item
Summary
Modality
Activation
interference

Treatments

2 items
Summary
stimulation
chemical
Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours
stimulation
protein
Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

Attribution

Award
HG012076
Principal Investigator(s)
Contact P.I.
Ansuman Satpathy
Lab
Ansuman Satpathy, Stanford