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HCT116 — IGVFSM9911OSCX
HCT116 — IGVFSM9911OSCX
released
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Summary
Homo sapiens (male) HCT116 cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
HCT116
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
IGVFDS7857SYCK
guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Sources
atcc
Aliases
jesse-engreitz:in-vitro-system_CRUDO_MYC
Attribution
Jesse Engreitz, Stanford
(
HG011972
—
Jesse Engreitz
)
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO0384OZRW
igvf:donor_of_HCT116
male
Homo sapiens
Origin Sample Of
2 items
Accession
Type
Sample Terms
Summary
IGVFSM3566GVBK
InVitroSystem
HCT116
Homo sapiens (male) HCT116 cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
IGVFSM3860UMMV
InVitroSystem
HCT116
Homo sapiens (male) HCT116 cell line treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Modifications
2 items
Summary
Modality
Activation
CRISPRi Sp-dCas9-KOX1-KRAB
interference
inactive AID system targeting RAD21
degradation
Documents
2 items
Page
Description
Type
Download
Preview
Generation of inducible CRISPRi line
plasmid map
addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Generation of inducible CRISPRi line
plasmid map
takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf