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Jurkat — IGVFSM9894RFCK

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This object is in preview status. Metadata is subject to change until this object is released.
Summary
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM4933EQTP FACS bin for 51-75% expression of IL2RA
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinC
Protocols

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

File Sets

2 items
prime editing Variant-EFFECTS targeting IL2RA: variant effects
  • jesse-engreitz:IL2RA_promoter_tiling_Jurkat-Stimulation_Variant-EFFECTS_analysis
Jesse Engreitz, Stanford
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
  • jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep2-BinC_AmpliconSequencing
Jesse Engreitz, Stanford

Modifications

1 item
Summary
Modality
prime editing

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford