Jurkat — IGVFSM9819UGFN

released
{
    "@context": "/terms/",
    "@id": "/in-vitro-systems/IGVFSM9819UGFN/",
    "@type": [
        "InVitroSystem",
        "Biosample",
        "Sample",
        "Item"
    ],
    "accession": "IGVFSM9819UGFN",
    "age": "unknown",
    "aliases": [
        "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinB"
    ],
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM9819UGFN](/in-vitro-systems/IGVFSM9819UGFN/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM9819UGFN/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "classifications": [
        "cell line"
    ],
    "construct_library_sets": [
        "/construct-library-sets/IGVFDS6377KNCH/"
    ],
    "creation_timestamp": "2023-11-30T22:31:43.573137+00:00",
    "demultiplexed_to": [],
    "donors": [
        "/human-donors/IGVFDO1080XFGV/"
    ],
    "file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS5248WHUK/",
            "accession": "IGVFDS5248WHUK",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinB_AmpliconSequencing"
            ],
            "assay_term": {
                "term_name": "cas mediated mutagenesis"
            },
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "status": "released",
            "summary": "cas mediated mutagenesis (Variant FlowFISH) integrating a guide (sgRNA) library targeting sequence variants in a genomic locus followed by DNA sequencing assay"
        },
        {
            "@id": "/analysis-sets/IGVFDS9162RHFP/",
            "accession": "IGVFDS9162RHFP",
            "aliases": [
                "jesse-engreitz:PPIF_promoter_8mer_insertion_Jurkat-NoStimulation_Variant-FlowFISH_analysis"
            ],
            "lab": {
                "title": "Jesse Engreitz, Stanford"
            },
            "status": "released",
            "summary": "primary analysis of Variant FlowFISH data"
        }
    ],
    "institutional_certificates": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "modifications": [
        {
            "@id": "/modifications/e8d549e6-cef0-482a-9deb-76d3b7ecaac3/",
            "modality": "prime editing",
            "status": "released",
            "summary": "CRISPR prime editing SpnCas9-M-MLV RT (PE2)"
        }
    ],
    "multiplexed_in": [],
    "notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\"",
    "origin_of": [],
    "parts": [],
    "pooled_in": [],
    "release_timestamp": "2023-12-20T22:54:32.335897+00:00",
    "sample_terms": [
        {
            "@id": "/sample-terms/EFO_0002796/",
            "term_name": "Jurkat"
        }
    ],
    "schema_version": "20",
    "sex": "male",
    "sorted_fractions": [],
    "sorted_from": {
        "@id": "/in-vitro-systems/IGVFSM1127DRPZ/",
        "accession": "IGVFSM1127DRPZ"
    },
    "sorted_from_detail": "FACS bin for 11-20% expression of PPIF",
    "sources": [
        {
            "@id": "/sources/atcc/",
            "title": "ATCC"
        }
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with a guide library",
    "taxa": "Homo sapiens",
    "uuid": "20da2406-3765-4bac-8c30-663eb57fad37",
    "virtual": false
}