{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM9390JSDH/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9390JSDH",
"age": "unknown",
"aliases": [
"jay-shendure:CRISPRa_VP64_K562_10Xlane2"
],
"audit": {},
"award": {
"@id": "/awards/HG011966/",
"component": "functional characterization"
},
"cellular_sub_pool": "10Xlane2",
"classifications": [
"cell line"
],
"construct_library_sets": [
"/construct-library-sets/IGVFDS7581DNXF/"
],
"creation_timestamp": "2023-12-01T23:55:58.541796+00:00",
"demultiplexed_to": [],
"donors": [
"/human-donors/IGVFDO9208RPQQ/"
],
"file_sets": [
{
"@id": "/auxiliary-sets/IGVFDS8929VNCP/",
"accession": "IGVFDS8929VNCP",
"aliases": [
"jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane2_CS1"
],
"lab": {
"title": "Jay Shendure, UW"
},
"status": "released",
"summary": "gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/measurement-sets/IGVFDS8535CXVX/",
"accession": "IGVFDS8535CXVX",
"aliases": [
"jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane2_exprs"
],
"assay_term": {
"term_name": "in vitro CRISPR screen using single-cell RNA-seq"
},
"lab": {
"title": "Jay Shendure, UW"
},
"status": "released",
"summary": "scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
{
"@id": "/analysis-sets/IGVFDS9078ZWQH/",
"accession": "IGVFDS9078ZWQH",
"aliases": [
"jay-shendure:analysis_set_CRISPRa_K562"
],
"lab": {
"title": "Jay Shendure, UW"
},
"status": "released",
"summary": "principal analysis of scCRISPR screen data"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"modifications": [
{
"@id": "/crispr-modifications/ee99bf66-2a5a-4ec6-8380-9434b259afd1/",
"modality": "activation",
"status": "released",
"summary": "CRISPRa Sp-dCas9-VP64"
}
],
"multiplexed_in": [],
"notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/e95d9da9-f77b-4532-8e1d-f76b594176b4/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_e95d9da9-f77b-4532-8e1d-f76b594176b4.",
"origin_of": [],
"part_of": "/in-vitro-systems/IGVFSM9778VZHD/",
"parts": [],
"pooled_in": [],
"publications": [
{
"@id": "/publications/20fcbdbb-5a47-41c6-b80b-e6dd0e8a8d3e/",
"publication_identifiers": [
"doi:10.1101/2023.03.28.534017"
]
}
],
"release_timestamp": "2024-08-13T20:47:07.426879+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002067/",
"term_name": "K562"
}
],
"schema_version": "23",
"sex": "female",
"sorted_fractions": [],
"sources": [
{
"@id": "/sources/jay-shendure/",
"title": "Jay Shendure, UW"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"summary": "K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with a guide library",
"taxa": "Homo sapiens",
"uuid": "176d17f8-d142-4cbd-a237-88a185b83cd0",
"virtual": false
}