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HepG2 — IGVFSM9172BOGO

released
Summary
Homo sapiens (male) HepG2 cell line modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Taxa
Homo sapiens
Description
Bulk samples; not sorted
Sources
Richard Sherwood, Brigham and Women's Hospital
Aliases
richard-sherwood:B4.2_18loci_Rep4_bulk_ivs
Attribution
Richard Sherwood, Brigham and Women's Hospital

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HepG2
male
Homo sapiens

File Sets

2 items
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
  • richard-sherwood:18loci_ABE_LDLuptake_bean_analysis_set
Richard Sherwood, Brigham and Women's Hospital
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
  • richard-sherwood:B4.2_18loci_Rep4_bulk_ms
Richard Sherwood, Brigham and Women's Hospital

Modifications

1 item
Summary
Modality
Activation
base editing

Sorted Fractions of Sample

4 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Bottom 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Bottom 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)
InVitroSystem
Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)