HCT116 — IGVFSM9116DXLY

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS0392OWNZ Guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
Taxa
Homo sapiens
Sources
Sorted From Sample
IGVFSM6219XAXK FACS bin E for 80-90% expression of CCND1
Aliases
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR1_S5
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR2_S101
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR3_S197
jesse-engreitz:in-vitro-system_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR4_S293

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

3 items
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR1_S5
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR2_S101
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR3_S197
  • jesse-engreitz:measurement_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR4_S293
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_PILOT_CCND1-Auxin6hrs-FF1
Jesse Engreitz, Stanford
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR1_S5
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR2_S101
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR3_S197
  • jesse-engreitz:auxiliary_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR4_S293
Jesse Engreitz, Stanford

Modifications

2 items
degradation
interference

Documents

Attribution