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HCT116 — IGVFSM9082URPW

released
Summary
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Sample Terms
Classification
cell line
Sex
male
Age
unknown
Construct Library Sets
  • IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci
Taxa
Homo sapiens
Sources
atcc
Sorted From Sample
IGVFSM8770MGOE FACS bin E for 80-90% expression of SSFA2
Aliases
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR1-Rep1_S29
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR2-Rep1_S35
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83
jesse-engreitz:in-vitro-system_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

File Sets

4 items
mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR1-Rep1_S29
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR2-Rep1_S35
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83
  • jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89
Jesse Engreitz, Stanford
CRISPR degradation, interference FlowFISH screen targeting ITPRID2: differential element quantifications
  • jesse-engreitz:analysis_set_CRUDO_SSFA2
Jesse Engreitz, Stanford
gRNA sequencing
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR1-Rep1_S29
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR2-Rep1_S35
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89
Jesse Engreitz, Stanford
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 3 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin6hrs-FF1
Jesse Engreitz, Stanford

Modifications

2 items
degradation
interference

Treatments

1 item
Term Name
Purpose
Type
Summary
activation
chemical
Treatment of 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours

Documents

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford