HCT116 — IGVFSM8987EBZC

released

Summary

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS1891GTLU guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM4652TCSQ FACS bin D for 55-65% expression of FAM3C

Aliases

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR1-Rep1_S124

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR2-Rep1_S130

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR3-Rep1_S178

jesse-engreitz:in-vitro-system_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR4-Rep1_S184

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

4 items

IGVFDS0713EITK

cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 2 more measurement sets

jesse-engreitz:auxiliary_sortparams_CRUDO_FAM3C_Auxin0hrs-FF2

Jesse Engreitz, Stanford

IGVFDS5239VQTN

CRISPR degradation, interference FlowFISH screen targeting FAM3C: differential element quantifications

jesse-engreitz:analysis_set_CRUDO_FAM3C

Jesse Engreitz, Stanford

IGVFDS5675MHUS

gRNA sequencing

jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR1-Rep1_S124
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR2-Rep1_S130
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR3-Rep1_S178
jesse-engreitz:auxiliary_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR4-Rep1_S184

Jesse Engreitz, Stanford

IGVFDS9829XFGT

mixed CRISPR FlowFISH screen sorted on the expression of FAM3C integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR1-Rep1_S124
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR2-Rep1_S130
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR3-Rep1_S178
jesse-engreitz:measurement_CRUDO_FAM3C_Auxin0hrs-FF2-BinD-PCR4-Rep1_S184

Jesse Engreitz, Stanford