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Jurkat — IGVFSM8530GMPU
Jurkat — IGVFSM8530GMPU
released
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Summary
Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library
Sample Terms
Jurkat
Classification
cell line
Sex
male
Age
unknown
Part of Sample
IGVFSM8505AKOK
Taxa
Homo sapiens
Sources
atcc
Aliases
jesse-engreitz:220829_8merInsertion-Jurkat-Amp_PPIF_promoter-BioRep4
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO1080XFGV
igvf:donor_of_Jurkat
male
Homo sapiens
Sample Parts
1 item
Accession
Type
Sample Terms
Summary
IGVFSM2633FEQZ
InVitroSystem
Jurkat
Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with a guide library
Modifications
1 item
Summary
Modality
CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)
prime editing
Treatments
2 items
Term Name
Purpose
Type
Summary
phorbol 13-acetate 12-myristate
stimulation
chemical
Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours
T-cell surface glycoprotein CD3 gamma chain
stimulation
protein
Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford