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GM25256 (WTC-11) — IGVFSM8464JMJS

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Summary
GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW283), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
Sample Terms
Classification
differentiated cell specimen
Sex
male
Cellular Sub Pool
subpool_LW283
Originated From Sample
IGVFSM6456YEWR
Age
unknown
Part of Sample
IGVFSM9036LWJT
Time Post Change
20 day
Cell Fate Change Protocol
protocol_NPC_Differentiation_Munshi_lab_1.pdf
Taxa
Homo sapiens
Description
differentiated WTC11 -dCAS9-KRAB-Lenti-sgRNA
Sources
Nikhil Munshi, UT
Submitter Comment
DIfferentiated WTC11 cell line (Lenti)
Aliases
nikhil-munshi:differentited_WTC11_TF_Perturb_Seq_Pilot_NPC_Lenti_LW283

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

File Sets

4 items
interference Perturb-seq: filtered feature barcode matrix, raw feature barcode matrix, sparse gene count matrix
  • gary-hon:WTC11_TF_Perturb_Seq_Pilot_NPC_Lenti_PrimaryAnalysis
Gary Hon, UT
cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_NPC_HTO_LW283
Gary Hon, UT
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_NPC_LW283
Gary Hon, UT
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • gary-hon:TF_Perturb_Seq_Pilot_Lenti_NPC_sgRNA_LW283
Gary Hon, UT

Modifications

1 item
Summary
Modality
interference

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT