HCT116 — IGVFSM8346JZPG

released

Summary

Homo sapiens (male) HCT116 cell line (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Sample Terms

HCT116

Classification

cell line

Sex

male

Age

unknown

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Taxa

Homo sapiens

Sources

atcc

Sorted From Sample

IGVFSM4717ECVY FACS bin A for 0-10% expression of KITLG

Aliases

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR1_S13

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR2_S19

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR4_S115

jesse-engreitz:in-vitro-system_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR3_S109

Attribution

Jesse Engreitz, Stanford

(

HG011972 — Jesse Engreitz

)

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

File Sets

3 items

IGVFDS4522DABT

cell sorting for CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:auxiliary_sortparams_CRUDO_KITLG-Auxin0hrs-FF2

Jesse Engreitz, Stanford

IGVFDS4649YECB

CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR1_S13
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR2_S19
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinA-PCR4_S115
jesse-engreitz:measurement_CRUDO_KITLG-Auxin0hrs-FF2-BinB-PCR3_S109

Jesse Engreitz, Stanford

IGVFDS6482AXWW

CRISPR interference, degradation FlowFISH screen targeting KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci